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Title of Journal: Cell Tissue Res

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Abbravation: Cell and Tissue Research

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Springer-Verlag

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DOI

10.1016/j.aca.2010.09.015

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1432-0878

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Isolation and characterization of neural stem cell

Authors: Kristen Rak Natalia V Wasielewski Andreas Radeloff Johannes Völkers Agmal Scherzed Sibylle Jablonka Rudolf Hagen Robert Mlynski
Publish Date: 2011/01/22
Volume: 343, Issue: 3, Pages: 499-508
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Abstract

Neural stem cells have been identified in multiple parts of the postnatal mammalian brain as well as in the inner ear No investigation of potential neural stem cells in the cochlear nucleus has yet been performed The aim of this study was to investigate potential neural stem cells from the cochlear nucleus by neurosphere assay and in histological sections to prove their capacity for selfrenewal and for differentiation into progenitor cells and cells of the neuronal lineage For this purpose cells of the cochlear nucleus of postnatal day 6 rats were isolated and cultured for generation of primary neurospheres Spheres were dissociated and cells analyzed for capacity for mitosis and differentiation Cell division was detected by cellcounting assay and BrdU incorporation Differentiated neural progenitor cells showed distinct labeling for Nestin and for Atoh1 Positive staining of ßIII Tubulin glial fibrillary acid protein GFAP and myelin basic protein MBP showed differentiation into neurons astrocytes and oligodendrocytes Furthermore Nestin and BrdUlabeled cells could also be detected in histological sections In conclusion the isolated cells from the cochlear nucleus presented all the features of neural stem cells cell division presence of progenitor cells and differentiation into different cells of the neuronal lineage The existence of neural stem cells may add to the understanding of developmental features in the cochlear nucleus

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