Authors: Morio Azuma Tsunehiro Suzuki Hiroshi Mochida Shigeyasu Tanaka Minoru Uchiyama Akiyoshi Takahashi Kouhei Matsuda
Publish Date: 2012/05/22
Volume: 350, Issue: 1, Pages: 167-176
Abstract
Somatolactin SL is a pituitary hormone belonging to the growth hormone/prolactin family of adenohypophyseal hormones In teleost fish SL is encoded by one or two paralogous genes namely SLα and β Our previous studies have revealed that pituitary adenylatecyclaseactivating polypeptide stimulates SL release from cultured goldfish pituitary cells whereas melaninconcentrating hormone suppresses this release As in other fish the goldfish possesses SLα and β So far however no useful means of detecting the respective SLs immunologically in this species has been possible In order to achieve this aim we raised rabbit antisera against synthetic peptide fragments deduced from the goldfish SLα and β cDNA sequences Using these antisera we observed adenohypophyseal cells showing SLα and βlike immunoreactivities in the goldfish pituitary especially the pars intermedia PI Several cells in the PI showed the colocalization of SLα and βlike immunoreactivities Then using singlecell polymerase chain reaction with laser microdissection we examined SLα and β gene expression in adenohypophyseal cells showing SLα or βlike immunoreactivity Among cultured pituitary cells we observed three types of cell those that possess transcripts of SLα β or both These results suggest a polymorphism of SLproducing cells in the goldfish pituitary
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