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Title of Journal: Cell Tissue Res

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Abbravation: Cell and Tissue Research

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Publisher

Springer Berlin Heidelberg

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DOI

10.1007/bf03159895

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ISSN

1432-0878

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Pluripotent stem cell transcription factors during

Authors: Juliana Malta da Cunha Adriana da CostaNeves Irina Kerkis Marcelo Cavenaghi Pereira da Silva
Publish Date: 2013/06/05
Volume: 353, Issue: 3, Pages: 435-441
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Abstract

Stem cells are capable of generating various cell lines and can be obtained from adult or embryonic tissues for clinical therapies Stem cells from deciduous dental pulp are among those that are easily obtainable from adult tissues and have been widely studied because of their ability to differentiate into a variety of cell lines in the presence of various chemical mediators We have analyze the expression of several proteins related to the differentiation and proliferative potential of cell populations that compose the tooth germ of human fetuses We evaluate 20 human fetuses of both genders After being paraffinembedded cap and bell stages of tooth germ development were subjected to immunohistochemistry for the following markers Oct4 Nanog Stat3 and Sox2 The studied antibodies showed nuclear or cytoplasmic immunnostaining within various anatomical structures and with various degrees of expression indicating the action of these proteins during tooth development We conclude that the interrelationship between these transcription factors is complex and associated with selfrenewal and cell differentiation Our results suggest that the expression of Oct4 Nanog Sox2 and Stat3 are related to differentiation in ameloblasts and odontoblastsOdontogenesis stages a Bud stage b Early cap stage c Cap stage d Early bell stage e Bell stage PG permanent tooth germ SR stellate reticulum P papillae IE inner enamel epithelium OE outer enamel epithelium En enamel Am ameloblasts D dentin JPEG 83 kb

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