Authors: Isabelle M Frey Isabel RubioAliaga Martina Klempt Eckhard Wolf Hannelore Daniel
Publish Date: 2006/04/04
Volume: 452, Issue: 3, Pages: 300-
Abstract
The peptide transporter PEPT2 mediates cellular uptake of di and tripeptides driven by an inwardly directed electrochemical proton gradient In mammals PEPT2 is found in a variety of organs such as kidney lung brain enteric nervous system and mammary gland Highest expression levels are observed in renal proximal tubules where PEPT2 contributes to reabsorption of filtered di and tripeptides To assess the physiological importance of the transporter in overall metabolism we have generated a Pept2 −/− mouse line that lacks a functional PEPT2 protein Here we present data on body weight organ weights and blood pressure Mice were then fed diets containing either 10 20 or 30 w/w protein and food and water intake rates as well as plasma and urine parameters were determined In spite of PEPT2 expression in a variety of tissues only subtle phenotypic changes were observed Male PEPT2 null mice displayed lower bodyweight and lower relative heart weight whereas relative kidney weight was lower in female Pept2 −/− mice No differences were found in blood pressure When fed diets with different protein contents Pept2 −/− mice adapted food intake to dietary protein content with higher consumption rates on low protein and reduced food intake rates on the high protein diet
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