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Title of Journal: Apoptosis

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Abbravation: Apoptosis

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Springer US

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DOI

10.1007/s40710-015-0088-0

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ISSN

1573-675X

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RNA interference against HPV16 E7 oncogene leads t

Authors: Ni Sima Wei Wang Debo Kong Dongrui Deng Qian Xu Jianfeng Zhou Gang Xu Li Meng Yunping Lu Shixuan Wang Ding Ma
Publish Date: 2007/12/01
Volume: 13, Issue: 2, Pages: 273-281
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Abstract

The simultaneous expression of human papillomavirus type 16 HPV16 E6 and E7 oncogenes is pivotal for malignant transformation and maintenance of malignant phenotypes Silencing these oncogenes is considered to be applicable in molecular therapies of human cervical cancer However it remains to be determined whether HPV16 E6 and E7 could be both silenced to obtain most efficient antitumor activity by using RNA interference RNAi technology Herein we designed a small interfering RNA siRNA targeting HPV16E7 region to degrade either E6 or truncated E6 E6 and E7 mRNAs and to simultaneously knockdown both E6 and E7 expression Firstly the sequence targeting HPV16E7 region was inserted into the shRNA packing vector pSIRENDNR yielding pSIREN16E7 to stably express corresponding shRNA HPV16transformed SiHa and CaSki cells were used as a model system RTPCR Western Blotting MTT assay TUNEL staining Annexin V apoptosis assay and flow cytometry were applied to examine the effects of pSIREN16E7 Our results indicated that HPV16E7 specific shRNA 16E7shRNA induced selective degradation of E6 and E7 mRNAs and proteins E6 silencing induced accumulation of cellular p53 and p21 In contrast E7 silencing induced hypophosphorylation of retinoblastoma Rb protein The loss of E6 and E7 reduced cell growth and ultimately resulted in massive apoptotic cell death selectively in HPVpositive cancer cells compared with the HPVnegative ones We demonstrated that 16E7shRNA can induce simultaneous E6 and E7 suppression and lead to striking apoptosis in HPV16related cancer cells by activating cellular p53 p21 and Rb Therefore RNAi using E7 shRNA may have the genespecific therapy potential for HPV16related cancers

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  1. Annexin II receptor induces apoptosis independent of Annexin II
  2. p73 modulates HIV-1 Tat transcriptional and apoptotic activities in human astrocytes
  3. Tandem DEDs and CARDs suggest novel mechanisms of signaling complex assembly
  4. Thymosin alpha 1 suppresses proliferation and induces apoptosis in breast cancer cells through PTEN-mediated inhibition of PI3K/Akt/mTOR signaling pathway
  5. Activation of mitogen-activated protein kinases is essential for hydrogen peroxide -induced apoptosis in retinal pigment epithelial cells
  6. APLP1 promotes dFoxO-dependent cell death in Drosophila
  7. Protective effect of Homer 1a on tumor necrosis factor-α with cycloheximide-induced apoptosis is mediated by mitogen-activated protein kinase pathways
  8. Lymphocyte-derived microparticles induce apoptosis of airway epithelial cells through activation of p38 MAPK and production of arachidonic acid
  9. Atorvastatin inhibits the apoptosis of human umbilical vein endothelial cells induced by angiotensin II via the lysosomal-mitochondrial axis
  10. The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage
  11. Ouabain-induced apoptosis and Rho kinase: a novel caspase-2 cleavage site and fragment of Rock-2
  12. Hypoxia-mimetic agents desferrioxamine and cobalt chloride induce leukemic cell apoptosis through different hypoxia-inducible factor-1α independent mechanisms
  13. Synchronized turbo apoptosis induced by cold-shock
  14. Sensitization of human bladder tumor cells to TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis with a small molecule IAP antagonist
  15. Nitrate tolerance aggravates postischemic myocardial apoptosis and impairs cardiac functional recovery after ischemia
  16. Shogaols at proapoptotic concentrations induce G 2 /M arrest and aberrant mitotic cell death associated with tubulin aggregation
  17. Gadolinium induced effects on mammalian cell motility, adherence and chromatin structure
  18. Radiosensitization of melanoma cells through combined inhibition of protein regulators of cell survival
  19. Insulin inhibits myocardial ischemia-induced apoptosis and alleviates chronic adverse changes in post-ischemic cardiac structure and function
  20. Novel antiapoptotic effect of TBX15: overexpression of TBX15 reduces apoptosis in cancer cells
  21. Apoptosis of human fibrosarcoma HT-1080 cells by epigallocatechin-3- O -gallate via induction of p53 and caspases as well as suppression of Bcl-2 and phosphorylated nuclear factor-κB
  22. Sulindac-derived reactive oxygen species induce apoptosis of human multiple myeloma cells via p38 mitogen activated protein kinase-induced mitochondrial dysfunction
  23. In vitro antiproliferative, pro-apoptotic, antimetastatic and anti-inflammatory potential of 2,4-diacteylphloroglucinol (DAPG) by Pseudomonas aeruginosa strain FP10
  24. Inducer-and cell type-specific regulation of antiapoptotic MCL1 in myeloid leukemia and multiple myeloma cells exposed to differentiation-inducing or microtubule-disrupting agents
  25. Fas/FasL pathway-mediated alveolar macrophage apoptosis involved in human silicosis
  26. Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells
  27. Role of Bim in apoptosis induced in H460 lung tumor cells by the spindle poison Combretastatin-A4
  28. Nanosecond pulsed electric fields modulate the expression of Fas/CD95 death receptor pathway regulators in U937 and Jurkat Cells
  29. Overexpression of CREB protein protects from tunicamycin-induced apoptosis in various rat cell types
  30. Cellular responses to endoplasmic reticulum stress and apoptosis
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