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Title of Journal: Apoptosis

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Abbravation: Apoptosis

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Springer US

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DOI

10.1007/978-1-4614-4989-8_43

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1573-675X

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Ouabaininduced apoptosis and Rho kinase a novel

Authors: Mustafa Ark Aysun Özdemir Belgin Polat
Publish Date: 2010/07/27
Volume: 15, Issue: 12, Pages: 1494-1506
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Abstract

Ouabain a specific Na+/K+ATPase inhibitor has recently been identified as a mammalian hormone Its elevated concentrations in human plasma have also been associated with pathogenesis of several diseases Recent studies have shown that ouabain induces aponecrotic cell death in a celltype and dosedependent manner However the exact mechanism of ouabaininduced cell death is not fully understood The Rho GTPase effectors Rho kinases1 and 2 Rock1 and Rock2 which play central roles in the organization of the actin cytoskeleton involve in several models of apoptosis In this study we investigated the possible involvement of Rocks in ouabaininduced human umbilical vein endothelial cell HUVEC apoptosis Ouabain treatment resulted in loss of cell–cell and cell–substratum adhesion and apoptotic blebbing Pretreatment of cells with Y27632 a specific Rock inhibitor resulted in the inhibition of celltocell detachment and formation of membrane blebs However Y27632 did not prevent ouabaininduced cell–substratum detachment Instead treatment with Y27632 actually accelerated this process Ouabain treatment induced cleavage of Rock1 and Rock2 which was prevented by caspase3 and caspase2 specific inhibitors zDEVDfmk and zVDVADfmk respectively Ouabaininduced Rock2 cleavage generated a fragment of approximately 140 kDa corresponding to the consensus sequence of caspase2 on the carboxy terminus of Rock2 Although it has been previously shown that Rock2 was cleaved by caspase2 we have identified here a novel cleavage site and fragment of Rock2 Our data indicate that ouabain induces both Rock1 and Rock2 cleavage via caspasedependent mechanisms and provide evidence that Rocks are involved in ouabaininduced celltocell detachment and apoptosis

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  1. Annexin II receptor induces apoptosis independent of Annexin II
  2. p73 modulates HIV-1 Tat transcriptional and apoptotic activities in human astrocytes
  3. Tandem DEDs and CARDs suggest novel mechanisms of signaling complex assembly
  4. Thymosin alpha 1 suppresses proliferation and induces apoptosis in breast cancer cells through PTEN-mediated inhibition of PI3K/Akt/mTOR signaling pathway
  5. Activation of mitogen-activated protein kinases is essential for hydrogen peroxide -induced apoptosis in retinal pigment epithelial cells
  6. APLP1 promotes dFoxO-dependent cell death in Drosophila
  7. Protective effect of Homer 1a on tumor necrosis factor-α with cycloheximide-induced apoptosis is mediated by mitogen-activated protein kinase pathways
  8. Lymphocyte-derived microparticles induce apoptosis of airway epithelial cells through activation of p38 MAPK and production of arachidonic acid
  9. Atorvastatin inhibits the apoptosis of human umbilical vein endothelial cells induced by angiotensin II via the lysosomal-mitochondrial axis
  10. The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage
  11. Hypoxia-mimetic agents desferrioxamine and cobalt chloride induce leukemic cell apoptosis through different hypoxia-inducible factor-1α independent mechanisms
  12. Synchronized turbo apoptosis induced by cold-shock
  13. Sensitization of human bladder tumor cells to TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis with a small molecule IAP antagonist
  14. Nitrate tolerance aggravates postischemic myocardial apoptosis and impairs cardiac functional recovery after ischemia
  15. Shogaols at proapoptotic concentrations induce G 2 /M arrest and aberrant mitotic cell death associated with tubulin aggregation
  16. Gadolinium induced effects on mammalian cell motility, adherence and chromatin structure
  17. Radiosensitization of melanoma cells through combined inhibition of protein regulators of cell survival
  18. Insulin inhibits myocardial ischemia-induced apoptosis and alleviates chronic adverse changes in post-ischemic cardiac structure and function
  19. Novel antiapoptotic effect of TBX15: overexpression of TBX15 reduces apoptosis in cancer cells
  20. Apoptosis of human fibrosarcoma HT-1080 cells by epigallocatechin-3- O -gallate via induction of p53 and caspases as well as suppression of Bcl-2 and phosphorylated nuclear factor-κB
  21. Sulindac-derived reactive oxygen species induce apoptosis of human multiple myeloma cells via p38 mitogen activated protein kinase-induced mitochondrial dysfunction
  22. In vitro antiproliferative, pro-apoptotic, antimetastatic and anti-inflammatory potential of 2,4-diacteylphloroglucinol (DAPG) by Pseudomonas aeruginosa strain FP10
  23. Inducer-and cell type-specific regulation of antiapoptotic MCL1 in myeloid leukemia and multiple myeloma cells exposed to differentiation-inducing or microtubule-disrupting agents
  24. Fas/FasL pathway-mediated alveolar macrophage apoptosis involved in human silicosis
  25. Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells
  26. Role of Bim in apoptosis induced in H460 lung tumor cells by the spindle poison Combretastatin-A4
  27. Nanosecond pulsed electric fields modulate the expression of Fas/CD95 death receptor pathway regulators in U937 and Jurkat Cells
  28. RNA interference against HPV16 E7 oncogene leads to viral E6 and E7 suppression in cervical cancer cells and apoptosis via upregulation of Rb and p53
  29. Overexpression of CREB protein protects from tunicamycin-induced apoptosis in various rat cell types
  30. Cellular responses to endoplasmic reticulum stress and apoptosis
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