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Title of Journal: Apoptosis

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Abbravation: Apoptosis

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Springer US

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DOI

10.1007/bf01982934

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ISSN

1573-675X

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Apoptosis of human fibrosarcoma HT1080 cells by e

Authors: Mi Hee Lee DongWook Han SuongHyu Hyon JongChul Park
Publish Date: 2010/10/21
Volume: 16, Issue: 1, Pages: 75-85
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Abstract

Animal tumor bioassays and in vitro cell culture systems have demonstrated that epigallocatechin3Ogallate EGCG the predominant catechin in green tea possesses antiproliferative and proapoptotic effects on various cancer cells and tumors In this study we investigated the effects of EGCG on cell growth cell cycle progression and apoptosis in human fibrosarcoma HT1080 cells The involvement of p53 Bcl2 Bax caspases and nuclear factorκB NFκB was examined as a mechanism for the anticancer activity of EGCG Timedependent intracellular trafficking of EGCG was also determined using fluorescein isothiocyanate FITCconjugated EGCG FITCEGCG Our data show that EGCG treatment caused dosedependent cell growth inhibition cell cycle arrest at the G0/G1 phase and DNA fragmentation suggesting the induction of apoptosis in HT1080 cells Immunoblot analysis revealed that the expression of p53 caspase7 and 9 as well as the ratio of Bax/Bcl2 protein increased significantly with higher EGCG concentrations and longer incubation times Moreover expression of phosphorylated NFκB/p65 in HT1080 cells was inhibited by EGCG treatment in a dosedependent manner while that of unphosphorylated NFκB/p65 remained unaffected Here we also reveal timedependent internalization of FITCEGCG into the cytosol of HT1080 cells and its subsequent nuclear translocation These results suggest that EGCG may interrupt exogenous signals directed towards genes involved in proliferation and cell cycle progression Taken together our data indicate that HT1080 apoptosis may be mediated through the induction of p53 and caspases by the prooxidant activity of internalized EGCG as well as suppression of Bcl2 and phosphorylated NFκB by the antioxidant activity of EGCGThis research was partly supported by the Korea Healthcare Technology RD Project Ministry for Health Welfare Family Affairs Republic of Korea A085136 and by a grant from the Fundamental RD Program for Core Technology of Materials funded by the Ministry of Knowledge Economy Republic of Korea K0006028

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  2. p73 modulates HIV-1 Tat transcriptional and apoptotic activities in human astrocytes
  3. Tandem DEDs and CARDs suggest novel mechanisms of signaling complex assembly
  4. Thymosin alpha 1 suppresses proliferation and induces apoptosis in breast cancer cells through PTEN-mediated inhibition of PI3K/Akt/mTOR signaling pathway
  5. Activation of mitogen-activated protein kinases is essential for hydrogen peroxide -induced apoptosis in retinal pigment epithelial cells
  6. APLP1 promotes dFoxO-dependent cell death in Drosophila
  7. Protective effect of Homer 1a on tumor necrosis factor-α with cycloheximide-induced apoptosis is mediated by mitogen-activated protein kinase pathways
  8. Lymphocyte-derived microparticles induce apoptosis of airway epithelial cells through activation of p38 MAPK and production of arachidonic acid
  9. Atorvastatin inhibits the apoptosis of human umbilical vein endothelial cells induced by angiotensin II via the lysosomal-mitochondrial axis
  10. The c-Abl tyrosine kinase stabilizes Pitx1 in the apoptotic response to DNA damage
  11. Ouabain-induced apoptosis and Rho kinase: a novel caspase-2 cleavage site and fragment of Rock-2
  12. Hypoxia-mimetic agents desferrioxamine and cobalt chloride induce leukemic cell apoptosis through different hypoxia-inducible factor-1α independent mechanisms
  13. Synchronized turbo apoptosis induced by cold-shock
  14. Sensitization of human bladder tumor cells to TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis with a small molecule IAP antagonist
  15. Nitrate tolerance aggravates postischemic myocardial apoptosis and impairs cardiac functional recovery after ischemia
  16. Shogaols at proapoptotic concentrations induce G 2 /M arrest and aberrant mitotic cell death associated with tubulin aggregation
  17. Gadolinium induced effects on mammalian cell motility, adherence and chromatin structure
  18. Radiosensitization of melanoma cells through combined inhibition of protein regulators of cell survival
  19. Insulin inhibits myocardial ischemia-induced apoptosis and alleviates chronic adverse changes in post-ischemic cardiac structure and function
  20. Novel antiapoptotic effect of TBX15: overexpression of TBX15 reduces apoptosis in cancer cells
  21. Sulindac-derived reactive oxygen species induce apoptosis of human multiple myeloma cells via p38 mitogen activated protein kinase-induced mitochondrial dysfunction
  22. In vitro antiproliferative, pro-apoptotic, antimetastatic and anti-inflammatory potential of 2,4-diacteylphloroglucinol (DAPG) by Pseudomonas aeruginosa strain FP10
  23. Inducer-and cell type-specific regulation of antiapoptotic MCL1 in myeloid leukemia and multiple myeloma cells exposed to differentiation-inducing or microtubule-disrupting agents
  24. Fas/FasL pathway-mediated alveolar macrophage apoptosis involved in human silicosis
  25. Mitochondrion-mediated apoptosis induced by Rana grylio virus infection in fish cells
  26. Role of Bim in apoptosis induced in H460 lung tumor cells by the spindle poison Combretastatin-A4
  27. Nanosecond pulsed electric fields modulate the expression of Fas/CD95 death receptor pathway regulators in U937 and Jurkat Cells
  28. RNA interference against HPV16 E7 oncogene leads to viral E6 and E7 suppression in cervical cancer cells and apoptosis via upregulation of Rb and p53
  29. Overexpression of CREB protein protects from tunicamycin-induced apoptosis in various rat cell types
  30. Cellular responses to endoplasmic reticulum stress and apoptosis
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