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Title of Journal: Amino Acids

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Abbravation: Amino Acids

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Springer Vienna

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DOI

10.1016/0304-4076(81)90080-4

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ISSN

1438-2199

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Near infrared spectroscopy compared to liquid chro

Authors: Christine H Petter Nico Heigl Stefan Bachmann Verena A C HuckPezzei Muhammad NajamulHaq Rania Bakry Andreas BernkopSchnürch Günther K Bonn Christian W Huck
Publish Date: 2007/12/20
Volume: 34, Issue: 4, Pages: 605-616
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Abstract

Peptide interaction is normally monitored by liquid chromatography LC liquid chromatography coupled to mass spectrometry LCMS mass spectrometric MS methods such as MALDITOF/MS or capillary electrophoresis CE These analytical techniques need to apply either high pressure or high voltages which can cause cleavage of newly formed bondages Therefore near infrared reflectance spectroscopy NIRS is presented as a rapid alternative to monitor the interaction of glutathione and oxytocin simulating physiological conditions Thereby glutathione can act as a nucleophile with oxytocin forming four new conjugates via a disulphide bondage Liquid chromatography coupled to UV LCUV and mass spectrometry via an electrospray ionisation interface LCESIMS resulted in a 82 and a 78 degradation of oxytocin at pH 3 and a 5 and a 7 degradation at pH 65 Capillary electrophoresis employing UVdetection CEUV showed a 44 degradation of oxytocin LC and CE in addition to the NIRS are found to be authentic tools for quantitative analysis Nevertheless NIRS proved to be highly suitable for the detection of newly formed conjugates after separating them on a thin layer chromatography TLC plate The recorded fingerprint in the near infrared region allows for a selective distinct qualitative identification of conjugates without the need for expensive instrumentation such as quadrupole or MALDITOF mass spectrometers The performance of the established NIRS method is compared to LC and CE its advantages are discussed in detail

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