Authors: Zhang Quan Xie Han Zhang Ye Yu Chenzhong Chen Wenjun
Publish Date: 2011/08/21
Volume: 62, Issue: 1, Pages: 119-123
Abstract
To investigate whether the mesoporous bioactive glass MBG exerts any in vitro bioactivity on rat osteoblasts and the potential regulatory mechanism of this bioactivity Rat osteoblasts were incubated in the presence and absence control of ionic dissolution product of MBG in minimal essential medium The osteoblast proliferation and differentiation were measured using MTT and alkaline phosphatase methods The IGFII mRNA expression in osteoblasts was measured by RTPCR IGFII protein and IGFBP were measured by ELISA The level of alkaline phosphatase activity was increased to 125 of control Expression of IGFII mRNA was increased to 125 of control There was a 175 and 237 increase in the concentration of unbound IGFII protein and IGFBP respectively in the conditioned media of treated osteoblasts The osteoblast proliferation was 92 of control The ionic dissolution product of MBG was able to promote the differentiation of osteoblasts probably by inducing IGFII expression at both mRNA and protein level
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