Authors: Yasuyuki Morita Taichi Mukai Yang Ju Sachi Watanabe
Publish Date: 2012/10/23
Volume: 66, Issue: 1, Pages: 73-80
Abstract
In the present study we evaluated whether stem celltotenocyte differentiation could be evaluated via measurement of the mechanical properties of the cell We used mechanical uniaxial cyclic stretching to induce the differentiation of human bone marrow mesenchymal stem cells into tenocytes The cells were subjected to cyclic elongation of 10 or 15 at a cyclic frequency of 1 Hz for 24 or 48 h and differentiation was assessed by realtime PCR rtPCR determination of messenger RNA expression levels for four commonly used markers of stem celltotenocyte differentiation type I collagen type III collagen tenascinC and scleraxis The rtPCR results showed that cells subjected to 10 cyclic elongation for 24 or 48 h differentiated into tenocytes Atomic force microscopy AFM was then used to measure the force curves around the cell nuclei and the AFM data were used to calculate the elastic moduli of the cell surfaces The elastic modulus values of the control nonstretched cells differed significantly from those of cells stretched at 10 for 24 or 48 h P 001 Confocal fluorescence microscopic observations of actin stress fibers suggested that the change in elastic modulus was ascribable to the development of the cellular cytoskeleton during the differentiation process Therefore we conclude that the atomic force microscopic measurement of the elastic modulus of the cell surface can be used to evaluate stem celltotenocyte differentiation
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