Authors: Deniz Say Sahin Cemil Tumer Cemil Demir M Murat Celik Mustafa Celik Edip Ucar Ramazan Gunesacar
Publish Date: 2012/09/30
Volume: 65, Issue: 2, Pages: 243-247
Abstract
Leptin is a protein hormone which plays a critical role in the regulation of both bodyweight through reducing food intake and stimulating energy expenditure Several polymorphisms in leptin gene LEP which encodes for leptin have been described However its association with obesity is still controversial Therefore in the present study we aimed to investigate whether LEP c2548 GA polymorphism was associated with serum leptin levels lipid parameters and body mass index in Turkish obese patients Fortyseven obese patients and 48 healthy individuals were included in the study Blood samples were collected for DNA extraction LEP c2548 GA polymorphism were detected using polymerase chain reaction–restriction fragment length polymorphism technique Serum leptin levels and lipid parameters were measured by ELISA and enzyme colorimetric assay techniques respectively GA or AA genotypes and A allele carrier frequencies of the c2548 GA polymorphism in the LEP were higher in obese 383 340 and 723 when compared with controls 146 125 and 271 p = 0011 0016 and 0002 respectively On the other hand AA or AG genotypes were also related to increased serum leptin levels p 0001 and body mass index p 00001 All these consequences showed that LEP 2548 AA or AG genotypes are important predictors for increased levels of leptin and BMI in Turkish obese patients and it may be a useful marker for obesity risk in our population
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