Authors: Zhibo Yan Weijie Chen Shaozhuang Liu Guangyong Zhang Dong Sun Sanyuan Hu
Publish Date: 2012/01/18
Volume: 22, Issue: 3, Pages: 493-501
Abstract
Ileal transposition IT as one of the effective treatments for nonobese type 2 diabetes mellitus has been widely investigated However the mechanisms underlying profound improvements in glucose homeostasis are still uncertain Our objective was to explore the myocardial insulin signal transduction and glucose disposal in nonobese type 2 diabetes mellitus rats after IT surgeryAdult male Goto–Kakizaki GK rats or Sprague–Dawley SD rats were randomly assigned to diabetic IT diabetic shamIT and nondiabetic control SD groups Food intake body weight fasting plasma glucose insulin tolerance and serum glucagonlike peptide1 GLP1 were measured Subsequently the myocardial glucose uptake and the protein levels of insulin receptorbeta IRβ phosphorylated IRβ insulin receptor substrate 1 IRS1 phosphorylated IRS1 and IRS1associated phosphatidylinositol3 kinase PI3K from myocardial cell lysates were evaluated We also assessed the expression of glucose transporter 4 GLUT4 in both skeletal muscle and myocardial cell lysatesCompared to sham operations within 6 months IT surgery for GK rats did 1 result in less food intake and reduced body weight gain over time 2 improve plasma glucose homeostasis with increased serum GLP1 secretion and myocardial glucose uptake 3 increase protein expression of insulin signaling pathway including IRβ IRS1 and their phosphorylation levels and IRS1associated PI3K in the myocardium and 4 enhance the protein levels of membrane GLUT4 in skeletal muscle and myocardium
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