Authors: Bullerjahn GS Benzinger Rolf H
Publish Date: 1984/05/01
Volume: 22, Issue: 3, Pages: 183-187
Abstract
Transposon Tn501 which encodes resistance to mercuric ions was introduced into Rhizobium japonicum 110 and 31 by conjugal transfer The transposon donor plasmid pMD100 was able to mobilize into R japonicum but could not be maintained Hg2+resistant colonies were recovered at a frequency of 19 × 10−8/recipient for strain 110 and 17 × 10−7/recipient for strain 31 Presence of Tn501 in Hgresistant isolates was verified by Southern analysis and demonstrating transposition of Hg resistance Transposon mutagenesis has been used to generate auxotrophic mutations at low frequency
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