Authors: Sugii Shunji Horiguchi Yasuhiko Uemura Takashi
Publish Date: 1986/04/01
Volume: 34, Issue: 2, Pages: 205-209
Abstract
Shunji Sugii Yasuhiko Horiguchi Takashi Uemura Hemagglutinating activity of trypsinized Clostridium perfringens enterotoxin FEMS Microbiology Letters Volume 34 Issue 2 April 1986 Pages 205–209 https//doiorg/101111/j157469681986tb01405xThe hemagglutinating activity of Clostridium perfringens enterotoxin CPE was studied after trypsin treatment Untreated CPE did not show any hemagglutinating activity to human type A B and O sheep chicken horse guineapig or rabbit erythrocytes Trypsinized CPE resulted in a more than 100fold increase in hemagglutinating activity with rabbit erythrocytes only Other erythrocytes and trypsinized rabbit erythrocytes were not agglutinated at all The hemagglutinating activity of CPE was also found on treatment with a lysinespecific proteinase On the other hand trypsinized CPE did not significantly increase the cytotoxic and enterotoxic activities The binding reaction between trypsinized and rabbit erythrocytes was not inhibited by any mono di or polysaccharides glycoproteins or ganglioside mixtures These results suggest that the hydrolysis of bonds involving lysine residues is mainly required for hemagglutinating activity and that the receptor for trypsinized CPE on rabbit erythrocytes is probably the protein moiety
Keywords: