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Title of Journal: FEMS Microbiol Lett

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Abbravation: FEMS Microbiology Letters

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Narnia

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DOI

10.1007/s12028-012-9676-2

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0378-1097

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Nucleotide sequence of Clostridium difficile toxin

Authors: Wren Brendan W Clayton Christopher L Tabaqchali Soad
Publish Date: 1990/06/01
Volume: 70, Issue: 1, Pages: 1-6
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Abstract

Brendan W Wren Christopher L Clayton Soad Tabaqchali Nucleotide sequence of Clostridium difficile toxin A gene fragment and detection of toxigenic strains by polymerase chain reaction FEMS Microbiology Letters Volume 70 Issue 1 June 1990 Pages 1–6 https//doiorg/101111/j157469681990tb03766xA 1947 base pair bp fragment of the toxin A gene of Clostridium difficile was sequenced A continuous open reading frame was found which contained 4 distinct groups of repeat nucleotide sequence with 88 to 100 identity within each group The arrangement of the groups A 81 bp B C and D 63 bp was ABCCCDABCDDABCCCDABCCDABCDABC Based on nucleotide sequence data from the C repeat group a pair of oligonucleotide primers were synthesised and used in the polymerase chain reaction PCR to amplify fragments from the toxin A gene Several products of multiples of 63 bp length were amplified for all 33 toxigenic C difficile strains tested in contrast to the 12 nontoxigenic strains tested which failed to amplify any product This rapid technique is of potential use in the specific identification of toxigenic C difficile strains in mixed culture and from clinical specimens

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References

citation author=Price SB citation author=Phelps CJ citation author=Wilkins TD citation author=Johnson JJ citation journal title=Curr Microbiol citation year=1987 citation volume=16 citation pages=8286

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