Authors: Duffey Paul S Guthertz Linda S
Publish Date: 1992/08/01
Volume: 95, Issue: 1, Pages: 27-36
Abstract
Paul S Duffey Linda S Guthertz Mycobacterium avium and Mycobacterium intracellulare chromatotypes defined by curvilinear gradient HPLC of mycolic acids FEMS Microbiology Letters Volume 95 Issue 1 August 1992 Pages 27–36 https//doiorg/101111/j157469681992tb05338xSeventynine strains of Mycobacterium avium complex bacteria MAC previously characterized by genetic probe analysis were assayed using two methods of reverse phase highperformance liquid chromatography HPLC that employed curvilinear gradients Although different in column length and cycle time the methods produced equivalent results yielding seven distinct chromatographic patterns chromatotypes of M avium and M intracellulare based on the ratio of mycolate concentrations in the late vs the middle of three peak clusters LM ratio The M avium strains n= 36 were assigned to chromototypes I through 4 LM ratios 3 and the M intracellulare strains n= 24 to chromototypes 5 through 7 LM ratios 4 Of 18 Mycobacterium‘X’ strains seven resembled M avium seven others resembled M intracellular and four were intermediate between M avium and M intracellulare
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