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Title of Journal: FEMS Microbiol Lett

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Abbravation: FEMS Microbiology Letters

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Narnia

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DOI

10.1002/chin.199230035

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0378-1097

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Combined influence of magnesium concentration and

Authors: Riedel KHJ Wingfield BD Britz TJ
Publish Date: 1992/04/01
Volume: 92, Issue: 1, Pages: 69-71
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Abstract

KHJ Riedel BD Wingfield TJ Britz Combined influence of magnesium concentration and polymerase chain reaction specificity enhancers FEMS Microbiology Letters Volume 92 Issue 1 April 1992 Pages 69–71 https//doiorg/101111/j157469681992tb05236xThe importance of an optimal magnesium concentration 65 mM as well as the inclusion of a specific polymerase chain reaction enhancer to obtain the correct fragment after amplification was examined during this study In contrast to tetramethylammonium chloride dimethyl sulphoxide did not enhance the specificity of amplification at different magnesium concentrations but rather altered the specificity resulting in the amplification of a larger fragment

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References

citation author=Charfreitag O citation author=Stackebrandt E citation journal title=J Gen Microbiol citation year=1989 citation volume=135 citation pages=20652070

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