Authors: Isaacson Richard E Start Gail L
Publish Date: 1992/01/01
Volume: 90, Issue: 2, Pages: 141-146
Abstract
Evaluation of 9 wildtype K99 positive strains of Escherichia coli showed that each had a plasmid of approximately 878 kb that hybridized with two DNA probes specific for K99 genes The K99 reference plasmid from E coli also is 878 kb Each of these strains had a conserved 715kb Bam HI fragment that also hybridized to these probes Several K99 negative mutants and three 3P− strains also contained K99 plasmids as well as the 715kb Bam HI fragment These results suggest that there is a conservation in size of the K99 plasmids of diverse strains
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