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Title of Journal: FEMS Microbiol Lett

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Abbravation: FEMS Microbiology Letters

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Narnia

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DOI

10.1016/0305-750x(88)90216-1

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0378-1097

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Rapid identification of bacteria of the Comamonada

Authors: Vaneechoutte Mario Rossau Rudi De Vos Paul Gillis Monique Janssens Danielle Paepe Noella De Rouck Ann Fiers Tom Claeys Geert Kersters Karel
Publish Date: 1992/06/01
Volume: 93, Issue: 3, Pages: 227-233
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Abstract

Mario Vaneechoutte Rudi Rossau Paul De Vos Monique Gillis Danielle Janssens Noella Paepe Ann De Rouck Tom Fiers Geert Claeys Karel Kersters Rapid identification of bacteria of the Comamonadaceae with amplified ribosomal DNArestriction analysis ARDRA FEMS Microbiology Letters Volume 93 Issue 3 June 1992 Pages 227–233 https//doiorg/101111/j157469681992tb05102xRibosomal rRNA gene fragments rDNA encompassing the 16S rDNA the 16S–23S rDNA spacer region and part of the 23S rDNA of 95 strains belonging to 13 welldescribed taxa of the eubacterial family comamonadaceae β subclass of the Proteobacteria or rRNA superfamily III were enzymatically amplified using conserved primers The fragments of approximately 2400 base pairs were subjected to restriction analysis Restriction fragment length patterns obtained with Hin fI enabled us to distinguish 9 of the 13 taxa studied Restriction with Cfo I was necessary to differentiate Acidovorax delafieldii from A temperans and Hydrogenophaga flava from H pseudoflava The results indicate that amplified rDNA restriction analysis is a simple and reliable tool for the identification of bacterial species

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References

citation author=Schwartz DC citation author=Saffran W citation author=Welsh J citation author=Haas R citation author=Goldenberg M citation author=Cantor CR citation title=Cold Spring Harbor Symp Quant Biol citation year=1982 citation volume=47 citation pages=189195

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  10. Introduction of the mercury transposon Tn501 into Rhizobium japonicum strains 31 and 110
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