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Title of Journal: FEMS Microbiol Lett

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Abbravation: FEMS Microbiology Letters

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Narnia

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DOI

10.1016/0165-0378(81)90089-9

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0378-1097

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A cloned 23S rRNA gene fragment of Bacillus subtil

Authors: Kraus Josef Ludwig Wolfgang Schleifer Karl Heinz
Publish Date: 1986/01/01
Volume: 33, Issue: 1, Pages: 89-93
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Abstract

Josef Kraus Wolfgang Ludwig Karl Heinz Schleifer A cloned 23S rRNA gene fragment of Bacillus subtilis and its use as a hybridization probe of conserved character FEMS Microbiology Letters Volume 33 Issue 1 January 1986 Pages 89–93 https//doiorg/101111/j157469681986tb01218xA subclone of plasmid p14B8 containing the major part of a 23S rRNA gene of Bacillus subtilis was constructed and designated pJK1 Labeled plasmid pJK1 could be used as a DNA probe with conserved gene sequences DNADNA hybridization experiments between filterbound DNA from various bacteria and labeled pJK1 showed a good correlation between oligonucleotide sequence analysis of 16S rRNA and DNA homology values Application of suboptimal or stringent hybridization conditions and an additional short incubation under the same conditions following hybridization yielded the best data for differentiating organisms related to B subtilis from less or nonrelated bacteria

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References

citation author=Schmeckpeper BJ citation author=Smith KD citation journal title=Biochemistry citation year=1972 citation volume=11 citation pages=13191326

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  12. Characterization of glucoamylase from Aspergillus terreus 4
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