Plasmid profiles and restriction fragment length p

Authors: Laguerre Gisèle Mazurier Sylvie Isabelle Amarger Noëlle

Publish Date: 1992/06/01

Volume: 101, Issue: 1, Pages: 17-26

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Abstract

Gisèle Laguerre Sylvie Isabelle Mazurier Noëlle Amarger Plasmid profiles and restriction fragment length polymorphism of Rhizobium leguminosarum bv viciae in field populations FEMS Microbiology Letters Volume 101 Issue 1 June 1992 Pages 17–26 https//doiorg/101111/j157469681992tb05757x56 isolates of Rhizobium leguminosarum biovar viciae from one field were characterized by analysis of plasmid profile total DNA restriction pattern and restriction fragment length polymorphism RFLP of 2 chromosomal regions and of symbiotic Sym plasmid Different levels of similarity exist in patterns generated by the different techniques At the level of partial similarity these techniques give comparable results for more than 80 of the isolates with the exception of RFLP profiling with the Sym probe Analysis at this level allows the grouping of the isolates that have most of their nonSym genome similarly organized At the level of total similarity the techniques are no more equivalent and provide complementary information on possible evolution of the different elements of the genome identified by each specific technique The nonSym plasmids defining classes were strongly associated with specific chromosomal backgrounds In contrast variations in Sym plasmids were not related with variations in the remaining genome Host range towards chromosomes was variable among the Sym plasmids which may reflect plasmid transfer between strains

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