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Title of Journal: FEMS Microbiol Lett

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Abbravation: FEMS Microbiology Letters

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Narnia

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DOI

10.1007/978-1-4615-0187-9_2

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0378-1097

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Cloning of the genes encoding urease from Proteus

Authors: Mörsdorf Gerhard Kaltwasser Heinrich
Publish Date: 1990/01/01
Volume: 66, Issue: 1-3, Pages: 67-73
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Abstract

Gerhard Mörsdorf Heinrich Kaltwasser Cloning of the genes encoding urease from Proteus vulgaris and sequencing of the structural genes FEMS Microbiology Letters Volume 66 Issue 13 January 1990 Pages 67–73 https//doiorg/101111/j157469681990tb03974xA fragment of chromosomal DNA from Proteus vulgaris encoding urease was cloned and expressed in Escherchia coli A 3 kbp region was sequenced and revealed three open reading frames with homology to jack Canavalia ensiformis urease The smallest protein 11 kDa was homologous to the Nterminus of the plant enzyme and the largest polypeptide 61 kDa corresponded to the Cterminus The large protein contained conserved regions and a cysteine residue which is known to be catalytically important in the plant enzyme A protein of 12 kDa showed homology to residues 132 to 237 of jack bean urease

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References

citation author=Collins CM citation author=Falkow S citation journal title=J Bacteriol citation year=1988 citation volume=170 citation pages=10411045

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