Journal Title
Title of Journal: Comp Clin Pathol
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Abbravation: Comparative Clinical Pathology
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Publisher
Springer London
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Authors: Omar M E AbdelSalam Yasser Ashry Khadrawy Eman R Youness Nadia A Mohammed Rehab Fawzy AbdelRahman Jihan Seid Hussein Nermeen Shafee
Publish Date: 2013/09/03
Volume: 23, Issue: 5, Pages: 1457-1467
Abstract
We investigated the effect of a single injection of rotenone into the striatum on the development of oxidative stress nigrostriatal cell injury and motor alterations in rats Rotenone 1 3 5 and 9 mM 5 μL/rat or the vehicle dimethyl sulfoxide was injected into the right striatum Control rats received the vehicle only Rats were allowed to recover from the operation and were tested for behavioural changes on 7th and 30th days after rotenone injection Biochemical markers of oxidative stress including malondialdehyde MDA reduced glutathione GSH nitric oxide paraoxonase 1 PON1 activity and Q10 enzyme as well as monoamine neurotransmitters in the brain were determined after 30 days of rotenone treatment Histopathology and tyrosine hydroxylase immunohistochemistry were also performed Results Intrastriatal injection of rotenone at 9 mM caused immediate death of rats No mortality was observed with the lower concentrations of the pesticide Rotenone at 1–5 mM resulted in increased brain oxidative stress in a dosedependent manner MDA increased by 235–649 while GSH decreased by 204–241 in the contralateral cerebral hemisphere Nitric oxide increased by 202–417 in ipsilateral cortex PON1 activity decreased by 125–382 in ipsilateral cerebral cortex and by 312–653 in ipsilateral striatum respectively but coenzyme Q10 increased in the ipsilateral cortex by 21–263 There was decreased dopamine and serotonin in the ipsilateral striatum after rotenone injection Tyrosine hydroxylase immunoreactivity was markedly decreased in ipsilateral substantia nigra in the rotenonetreated in contrast to the vehicletreated rats Rotenone increased the number of degenerated cells in substantia nigra in a dosedependent manner It also caused depletion of pigment granules from cells Degenerative changes were also observed in the contralateral hippocampus and cortex especially after the highest dose of rotenone The number of spontaneous rears made during 30 min in the cylinder was decreased in both limbs the decrease being more evident in the ipsilateral side Thus a single intrastriatal injection of rotenone a caused a significant nigrostriatal degeneration and loss of dopamine and serotonin from the striatum b elicited cell degeneration in the hippocampus and cortex c induced oxidative stress and neuronal injury this latter effect of rotenone was not region specific and d the motor deficits decreased rearing activity occurred in both limbs
Keywords:
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