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Title of Journal: Comp Clin Pathol

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Abbravation: Comparative Clinical Pathology

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Springer London

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DOI

10.1016/0022-5096(68)90034-3

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1618-565X

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Humoral immune response in Emphasis Type="Italic"

Authors: Dayanne C Fernandes Silas F Eto Gustavo S Claudiano Paulo F Marcusso Flávia C F Vieira Marina T Shimada Thiago F A Silva Elaine C Souza Rogério Salvador Julieta R E Moraes Flávio R Moraes
Publish Date: 2014/07/09
Volume: 24, Issue: 3, Pages: 617-623
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Abstract

Understanding the functional mechanisms of the humoral and cellular immune responses among teleost broadens the possibilities for intervention and improvement of vaccination methods The aim of this study was to evaluate the dynamics of the humoral immune response hematological biochemical changes and electrophoretic profile of proteins in Oreochromis niloticus when submitted to antigen stimulation using sheep erythrocytes in a single dose and with booster doses Seventy O niloticus were distributed in two groups n = 35 one received a single dose and the other received two doses of antigen Evaluations were made at the following predetermined times zero preimmune ie before inoculation with the first injection of sheep erythrocytes negative control and on 7th 14th 21st and 28th days after the first immunization to evaluate the hemogram biochemistry and electrophoresis of proteins and direct hemagglutination Twentyeight days after antigen inoculation it was observed that there had been an increase in the serum antibody titer and fractions gamma globulins after second antigen stimulation The results suggest that teleost fish have the capacity to produce antibodies from a single stimulation and a second antibody production wave after second stimulation


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