Experimental infection with Emphasis Type="Italic

Authors: Aleksandro S Da Silva Gabriela Campigotto Tais R Sczesny Rafael Pazinato Kassio D Albani Vanderlei Klauck Rhayana K Grosskopf Josué P Topazio Gabriela M Galli Chrystian J Cazzarotto Rogério Ferreira Alexandre A Tonin Silvia G Monteiro Paulo H E Weiss Luiz C Miletti Marta L R Leal

Publish Date: 2016/04/23

Volume: 25, Issue: 4, Pages: 841-846

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Abstract

The aims of this study were to investigate Trypanosoma evansi venereal transmission in ewes and its presence in ram’s semen and hematological and biochemical alterations Three adult rams and six ewes were used as follows one uninfected ram was used as control R1 and two rams R2 and R3 were intravenously infected by T evansi trypomastigotes Semen samples were collected weekly for PCR analyses that have detected the presence of T evansi Blood samples were collected and it was observed that the number of red blood cells and hemoglobin concentration were reduced in infected rams on day 14 postinfection PI to T evansi when compared to uninfected ram Likewise the number of total leukocytes increased in infected rams due to the increase of lymphocytes and monocytes The values of total protein and globulin increased from day 14 PI in animals infected by T evansi but albumin levels were reduced for these animals on the same period Testosterone levels on days 21 28 and 35 PI were lower in rams infected by T evansi All rams served two ewes each 17 days PI All ewes had synchronized estrus and 45 days after service gestational diagnosis was performed by ultrasound confirming that all the ewes were pregnant Blood samples from all ewes were collected every 30 days for 5 months for serological assays and PCR All results from ewes were negative T evansi was detected in semen samples there was no parasitic venereal transmission to ewes and no fertility impairment

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