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Title of Journal: J Therm Anal Calorim

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Abbravation: Journal of Thermal Analysis and Calorimetry

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Springer Netherlands

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DOI

10.1007/bf03172975

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ISSN

1572-8943

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Highsensitivity modulation differential scanning

Authors: G V Kotelnikov S P Moiseeva T V Burova N V Grinberg A Ya Mashkevich A S Dubovik V Ya Grinberg
Publish Date: 2013/02/06
Volume: 114, Issue: 2, Pages: 531-536
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Abstract

Method of highsensitivity modulation differential scanning calorimetry was applied for investigation of the kinetically controlled irreversible thermal denaturation of the trypsin inhibitor from soybeans Kunitz inhibitor KI in diluted solution The measurements were carried out with a temperaturemodulation capillary nanocalorimeter designed and produced by the Institute of Biological Instrumentation of the RAS Pushchino Russia An algorithm of the experimental data processing and corresponding software were developed It was shown that the modulation nanocalorimetry allows one to obtain in one experiment the temperature dependence of the rate constant for irreversible protein denaturation The temperature dependence of the rate constant and the activation energy of the irreversible denaturation of Kunitz inhibitor were determined The obtained value of the activation energy E a = 206 ± 6 kJ mol−1 agrees with independent estimates of this kinetic parameter

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