Authors: Xiaoyan Xing Yanling Zhao Weijun Kong Guibo Sun Ying Wang Xiaobo Sun Xiaohe Xiao
Publish Date: 2015/03/21
Volume: 120, Issue: 1, Pages: 973-979
Abstract
Microcalorimetry is an important analytical tool continuously measuring the heat output generated in the physical chemical or biological processes Here the microcalorimetric method was used to evaluate the bioactivity differences of seven medicinal animal horns and shells on splenic lymphocytes The cells were placed in the 20mL glass ampoules containing RPMI1640 and the test drugs 10 mg mL−1 and the heat output and motility of the cells were examined with an isothermal microcalorimeter From the measured power–time curves some quantitative thermokinetic parameters were obtained By analyzing these parameters using principal component analysis two main parameters the maximum heatoutput power P m and total heat output Q were optimized through which the effects of the seven drugs on splenic lymphocytes were quickly evaluated 10 mg mL−1 of Cornu Cervi Pantotrichum showed stronger promotion effect than Cornu Cervi on the growth and proliferation of splenic lymphocytes whereas the other five horns and shells animal drugs including Cornu Saigae Tataricae Cornu Caprae Hircus Cornu Bubali Squama Manis and Carapax Trionycis at 10 mg mL−1 all inhibited the growth of splenic lymphocytes and Cornu Saigae Tataricae expressed the strongest inhibitory effect The result of hierarchical clustering analysis on the quantitative parameters indicated that the drugs with promotion effect on splenic lymphocytes clustered into one group and the other drugs with inhibitory effect consisted of another group The results have highlighted the bioactivity differences of the seven horns and shells animal drugs referring to immune functions from the cell level providing meaningful references for the clinical use of the valuable drugs
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