Authors: Lediane Tomazi Carlos Fernando Mello Ana Paula Schöffer Bruna Amanda Girardi Pâmella Karina Santana Frühauf Maribel Antonello Rubin
Publish Date: 2016/01/14
Volume: 54, Issue: 1, Pages: 710-721
Abstract
Nmethyldaspartate NMDA receptor antagonists block morphineinduced conditioned place preference CPP Although polyamines are endogenous modulators of the NMDA receptor it is not known whether polyaminergic agents induce CPP or modulate morphineinduced CPP Here we examined whether polyamine ligands modify morphine CPP acquisition consolidation and expression Adult male albino Swiss mice received saline 09 NaCl intraperitoneally ip or morphine 5 mg/kg ip and were respectively confined to a black or a white compartment for 30 min for four consecutive days for CPP induction The effect of arcaine 3 mg/kg ip or spermidine 30 mg/kg ip respectively an antagonist and an agonist of the polyaminebinding site at the NMDA receptor on the acquisition consolidation and expression of morphine CPP was studied In those experiments designed to investigate whether spermidine prevented or reversed the effect of arcaine spermidine 30 mg/kg ip was administered 15 min before or 15 min after arcaine respectively Arcaine and spermidine did not induce CPP or aversion per se Arcaine 3 mg/kg ip impaired the acquisition consolidation and expression of morphine CPP Spermidine prevented the impairing effect of arcaine on the acquisition of morphine CPP but not the impairing effect of arcaine on consolidation or expression of morphine CPP These results suggest that arcaine may impair morphine CPP acquisition by modulating the polyaminebinding site at the NMDA receptor However the arcaineinduced impairment of consolidation and expression of morphine CPP seems to involve other mechanismsThis work was supported by the National Council for Scientific and Technological DevelopmentCNPq of Brazil grant numbers 306468/20140 307812/20146 and Fundação de Amparo à Pesquisa do Rio Grande do SulFAPERGS CFM and MAR are recipients of CNPq research productivity fellowships LT APS BAG and PKSF are recipients of “Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES” fellowships
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