Authors: Y S Cho N L Schiller H Y Kahng K H Oh
Publish Date: 2007/09/14
Volume: 55, Issue: 6, Pages: 501-506
Abstract
The purpose of this study was to characterize the cellular response and proteomic analysis of Escherichia coli exposed to tea polyphenols TPP extracted from Korean green tea Camellia sinensis L TPP showed a dosedependent bactericidal effect on E coli Analysis of cellmembrane fatty acids of E coli cultures treated with TPP identified unique changes in saturated and unsaturated fatty acids whereas scanning electron microscopic analysis demonstrated the presence of perforations and irregular rod forms with wrinkled surfaces in cells treated with TPP Twodimensional polyacrylamide gel electrophoresis of soluble protein fractions from E coli cultures exposed to TPP showed 17 protein spots increased or decreased by TPP Nine upregulated proteins were identified including GroEL and proteins involved in cellular defense such as GyrA RpoS SodC and EmrK whereas the expression of eight proteins was downregulated by exposure to TPP including proteins involved in carbon and energy metabolism such as Eno SdhA and UgpQ as well as those involved in aminoacid biosynthesis such as GltK and TyrB These results provide clues for understanding the mechanism of TPPinduced stress and cytotoxicity on E coli
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