Authors: Somayeh Ghahari Heshmatollah Alinezhad Ghorban Ali Nematzadeh Mahmood Tajbakhsh Robabeh Baharfar
Publish Date: 2017/03/02
Volume: 74, Issue: 4, Pages: 522-531
Abstract
Glycine max L Merrill soybean is a major leguminous crop cultivated globally as well as in Iran This study examines the chemical composition of soybean essential oil and evaluates the antioxidant and antimicrobial activities of seeds on various plant pathogens that commonly cause irreparable damages to agricultural crops The essential oil of soybean seeds was analyzed by gas chromatography coupled to mass spectrometry Antimicrobial activity was tested against 14 microorganisms including three grampositive five gramnegative bacteria and six fungi using disk diffusion method and the Minimum Inhibitory Concentration technique The soybean seeds were also subjected to screening for possible antioxidant activity by using catalase peroxidase superoxide dismutase and 22diphenyl1picrylhydrazyl DPPH assay Forty components were identified representing 9668 of the total oil The major constituents of the oil were carvacrol 1344 EE24decadienal 915 pallylanisole 565 pcymene 487 and limonene 475 The oil showed significant activity against Pseudomonas syringae subsp syringae Rathayibacter toxicus with MIC = 25 µg/mL and Pyricularia oryzae with MIC = 125 µg/mL In addition the free radical scavenging capacity of the essential oil was determined with an IC50 value of 16235 µg/mL Our results suggest that this plant may be a potential source of biocide for economical and environmentally friendly disease control strategies It may also be a good candidate for further biological and pharmacological investigations
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