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Title of Journal: Curr Microbiol

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Abbravation: Current Microbiology

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Springer US

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DOI

10.1016/j.bbaexp.2005.08.003

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1432-0991

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A Emphasis Type="Italic"nopA/Emphasis Deletion

Authors: WonSeok Kim Hari B Krishnan
Publish Date: 2013/10/10
Volume: 68, Issue: 2, Pages: 239-246
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Abstract

Sinorhizobium fredii USDA257 employs type III secretion system T3SS to deliver effector proteins into the host cells through pili The nopA protein is the major component of USDA257 pili The promoter region of USDA257 nopA possesses a well conserved tts box Serial deletion analysis revealed that the tts box is absolutely essential for flavonoid induction of nopA Deletion of nopA drastically lowered the number of nodules formed by USDA257 on cowpea and soybean cultivar Peking In contrast to the parental strain the USDA257 nopA mutant was able to form few nodules on soybean cultivars McCall and Williams 82 Light and transmission electron microscopy examination of these nodules revealed numerous starch grains both in the infected and uninfected cellsWe thank Julio Lorio and Jeong SunHyung for their technical assistance and Nathan Oehrle for reviewing this manuscript Microscopy was carried out at the Electron Microscopy Facility of the University of Missouri School of Veterinary Medicine Names are necessary to report factually on available data however either the University of Missouri or USDA neither guarantees nor warrants the standard of product and the use of the name by the University of Missouri or USDA implies no approval of the product to the exclusion of others that may be suitable

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