Authors: AiQun Yu JianChun Zhu Biao Zhang LaiJun Xing Mingchun Li
Publish Date: 2011/03/02
Volume: 62, Issue: 5, Pages: 1617-1622
Abstract
On the molecular and biochemical levels the effects of different carbon sources on biomass production fatty acid biosynthesis and gene expression of three desaturases were investigated in Mortierella alpina ATCC 16266 at a stationary phase which is an important filamentous fungus capable of producing various polyunsaturated fatty acids PUFAs The maximum mycelial biomass was achieved using sucrose as carbon source However the highest productivity of total lipids was shown to be no biomass associated In addition glucose was the preferred carbon source for the expression of three desaturase genes compared to others but the change at the corresponding fatty acid products level of these desaturase genes was not in accordance with the change measured at the mRNA level among those carbon sources that we utilized Significant discrepancies between the mRNA expression and the product abundance may indicate posttranscriptional regulatory mechanisms of these desaturases
Keywords: