Authors: Thamires Klein de Souza Scheila Silva Soares Lisianne Brittes Benitez Marilise Brittes Rott
Publish Date: 2017/03/03
Volume: 74, Issue: 5, Pages: 541-549
Abstract
The interactions that occur between bacteria and amoebae can give through mutual relations where both organisms benefit from the association or parasitic in which one organism benefits at the expense of the other When these organisms share the same environment it can result in some changes in the growth of organisms in adaptation patterns in morphology development or even in their ability to synthesize proteins and other substances In this study the interaction between Acanthamoeba polyphaga and Staphylococcus aureus MRSA was evaluated using a coculture model at different incubation times The results showed that 89 of amoebic cells remained viable after contact with the bacteria The bacterial isolate was visualized inside the amoeba through confocal microscopy and fluorescence for up to 216 h of cocultivation The lysate of amoebic culture increased the growth of S aureus MRSA and the effect of supernatant of culture inhibited bacterial growth over the incubation times suggesting that A polyphaga produced some metabolite that inhibited the growth of bacteria Moreover the encystment of the A polyphaga was increased by the bacteria presence The results show that A polyphaga and S aureus interaction may have an important influence on survival of both and specially indicate a possible effect on the metabolics characteristics each other
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