Authors: Sathish Prasad Mambatta Shankaranarayanan Pratibha Poorna Manasa Sailaja Buddhi Zareena Begum Sisinthy Shivaji
Publish Date: 2012/10/05
Volume: 66, Issue: 1, Pages: 64-71
Abstract
The abundance and diversity of chemotactic heterotrophic bacteria associated with Arctic cyanobacteria was determined The viable numbers ranged between 104 and 106 cell g−1 cyanobacterial biomass A total of 112 morphotypes representing 22 phylotypes based on their 16S rRNA sequence similarity were isolated from the samples All the phylotypes were Gramnegative with affiliation to the proteobacterial and bacteroidetes divisions Among the 22 phylotypes 14 were chemotactic to glucose Majority of the phylotypes were psychrotolerant showing growth up to 30 °C Representatives of Alphaproteobacteria the genus Flavobacterium and the gammaproteobacterial Alcanivorax sp were psychrophilic with growth at or below 18 °C A significant percentage of phylotypes were pigmented ~68 rich in unsaturated membrane fatty acids and tolerated pH values and NaCl concentrations between 50–80 and 015–10 M respectively The percentages of phylotypes producing extracellular coldactive enzymes at 4 °C were amylase 1818 lipase and urease 4545 caseinase 5909 and gelatinase 318 We would like to thank the Department of Biotechnology Council of Scientific and Industrial Research Government of India for financial support to SS SS would also like to thank NCAOR and Ministry of Earth Sciences Government of India for providing financial and logistic support for the trip to Arctic
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