Authors: Baolian Li Si Zhang Lijuan Long Sijun Huang
Publish Date: 2016/06/07
Volume: 73, Issue: 3, Pages: 409-418
Abstract
Three bacteriophages RD1410W101 RD1410Ws07 and DS1410Ws06 were isolated from the surface water of Sanya Bay northern South China Sea on two marine bacteria type strains of the Roseobacter lineage These phages have an isometric head and a short tail morphologically belonging to the Podoviridae family Two of these phages can infect four of seven marine roseobacter strains tested and the other one can infect three of them showing relatively broader host ranges compared to known N4like roseophages Onestep growth curves showed that these phages have similar short latent periods 1–2 h but highly variable burst sizes 27–341 pfu cell−1 Their complete genomes show high level of similarities to known N4like roseophages in terms of genome size G + C content gene content and arrangement The morphological and genomic features of these phages indicate that they belong to the N4likevirus genus Moreover comparative genomic analysis based on 43 N4like phages 10 roseobacter phages and 33 phages infecting other lineages of bacteria revealed a core genome of 18 genes shared by all the 43 phages and 38 genes shared by all the ten roseophages The 38 core genes of N4like roseophages nearly make up 70 of each genome in length Phylogenetic analysis based on the concatenated core gene products showed that our phage isolates represent two new phyletic branches suggesting the broad genetic diversity of marine N4like roseophages remainsWe thank our colleagues at the CAS Tropical Marine Biological Research Station in Hainan for their help in sampling We also thank Li Yinyin at Sun Yatsen University for her assistance in TEM This work was supported by the NSFC grants 41576126 SH 41206131 SH and 41230962 SZ
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