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Title of Journal: Curr Microbiol

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Abbravation: Current Microbiology

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Springer-Verlag

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DOI

10.1016/j.aca.2007.08.005

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1432-0991

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A Novel Degenerated Primer Pair Detects Diverse Ge

Authors: Yili Huang Yanhua Zeng Zhiliang Yu
Publish Date: 2013/03/13
Volume: 67, Issue: 2, Pages: 183-187
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Abstract

A novel degenerated primer set was designed to amplify acyl homoserine lactone AHL synthetase genes from members of the family Rhizobiaceae The primer set successfully amplified AHL synthetase genes from pure cultures of AHL producers from Rhizobiaceae but not from AHL producers out of the Rhizobiaceae family indicating the specificity of this primer set to the Rhizobiaceae family An inoculation experiment showed that the minimal detectable concentration of AHL producers from the soil was around 25 × 107 CFU/g soil When applying to environmental samples 7 and 14 different genotypes of AHL synthetase genes were identified in the rhizosphere of Glycine max and Vigna unguiculata respectively which revealed complicated and unknown AHLbased quorumsensing networks in the rhizosphere This is the first primer set that covers diverse AHL synthetase genes from different genera It will be a useful cultureindependent approach for better understanding of the ecological significance of QS in natural habitatsWe would like to thank Prof Lizhong Zhu and Prof Baoliang Chen for their technical support and constructive discussion during the experiments This work was financially supported by national natural science foundation of China 21137003 21077090 and natural science foundation of Zhejiang Province Y5100048 to Yili Huang

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