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Title of Journal: Curr Microbiol

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Abbravation: Current Microbiology

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Springer-Verlag

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DOI

10.1007/bf02478275

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1432-0991

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In Vitro and In Vivo Antioxidant Activity of Emph

Authors: Qian Shen Nan Shang Pinglan Li
Publish Date: 2010/12/04
Volume: 62, Issue: 4, Pages: 1097-1103
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Abstract

Several studies reported the antioxidant activity of bifidobacteria using assays in vitro In present study the in vitro and in vivo antioxidant activity of Bifidobacterium animalis 01 was investigated Culture supernatant intact cells and intracellular cellfree extracts of B animalis 01 were involved in this study The antioxidant assays in vitro included lipid peroxidation assay 11diphenyl2picrylhydrazyl DPPH assay hydroxyl radical • OH assay and superoxide anion textO 2 assay The antioxidant assays in vivo were conducted using mice model Activities of antioxidative enzymes malondialdehyde MDA content in serums and livers of aging mice were evaluated Monoamine oxidase MAO activity and lipofuscin level in brains of aging mice were also characterized Results showed that culture supernatant intact cells and intracellular cellfree extracts of B animalis 01 could effectively scavenge free radicals significantly enhance mice’s activities of antioxidative enzymes and reduce mice’s MDA content lipofuscin level and MAO activity Our results indicated that B animalis 01 has the potential to be developed into a dietary antioxidant supplements

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