Authors: Kanna Okayama Takeshi Honda Shigeaki Matsuda Tadashi Saito Masaya Kawase
Publish Date: 2010/06/01
Volume: 62, Issue: 1, Pages: 90-95
Abstract
During our research into the pathogenesis of Vibrio parahaemolyticus we noticed that the concentration of serum added to the tissue culture medium Dulbecco’s modified Eagle’s medium DMEM greatly affected its growth Using gel filtration column chromatography we clearly demonstrated that serum contains not only a bacterial growth inhibitor BGI but also a bacterial growth enhancer BGE for Vibrio parahaemolyticus Our data indicate that the BGI is transferrin whereas the BGE seems to be an undescribed small molecule molecular weight of 1000–3000 Da and is associated with magnesium and molybdenum ions BGE activity was not decreased by heat treatment at 60 or 100°C for 30 min and affected the growth rate of a wide range of Gramnegative and Grampositive bacteria The addition of EDTA into DMEM lowered the growth rate whereas the addition of BGE restored the growth activity This study suggests that sera contain a previously undescribed small BGE molecule
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