Authors: Chung J Chang Monique Garnier Leyla Zreik Victoria Rossetti Joseph M Bové
Publish Date: 2013/07/08
Volume: 27, Issue: 3, Pages: 137-142
Abstract
A xylemlimited bacterium resemblingXylella fastidiosa has been shown previously by electron mmcroscopy to be associated with citrus variegated chlorosis CVC a new disease of sweet organe tress in Brazil A bacterium was consistently cultured from plant tissues from CVC twigs of sweet orange trees but not from tissues of healthy trees on several cellfree media known to support the growth ofXylella fastidiosa Bacterial colonies typical ofX fastidiosa became visible on PW CS20 and PD2 agar media after 5 and 7–10 days of incubation respectively The cells of the CVC bacterium were rodshaped 14–3 μm in length and 02–04 μm in diameter with rippled walls An antiserum against an isolate 81b of the bacterium gave strong positive reactions to doubleantibodysandwich DAS enzymelinked immunosorbent assay ELISA with other cultured isolates from CVC citrus as well as with several type strains ofX fastidiosa This result indicates that the CVC bacterium is a strain ofX fastidiosa ELISA was also highly positive with all leaves tested from CVCaffected shoots Leaves from symptomless tress reacted negatively Sweet organe seedlings inoculated with a pure culture of the CVC bacterium supported multiplication of the bacterium which became systemic with 6 months after inoculation and could be reisolated from the inoculated seedlings Symptoms characteristic of CVC developed 9 months post inoculation
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