Authors: Ya Li ShuangHu Cai
Publish Date: 2010/09/19
Volume: 62, Issue: 2, Pages: 623-627
Abstract
Thirtysix strains numbered from PY01 to PY36 were isolated from six moribund Oreochromis niloticus The biochemical characteristics of all strains conformed to the species description of Aeromonas sobria on the basis of API 20E and Biolog GN system Furthermore gyrB sequence of strain PY36 was sequenced and showed high similarity 998 with A sobria in Genbank Antibioticresistance of strain PY36 was assessed by the KirbyBauer disk diffusion method and the results showed it was susceptible and moderately susceptible to 12 and 3 of the 19 antimicrobials tested Virulence of strain PY36 to juvenile tilapia was also tested and we found that LD50 was about 417 × 103 CFU per fish in intraperitoneal injection This is the first article to report that A sobria was the pathogenic agent of tailrot disease in juvenile tilapia A sobria was multiresistant to the most frequently used antimicrobial drugs in China so the antimicrobial resistance test should be carried out when these bacteria are isolated from biological samples in order to avoid therapeutic failures and spread of the pathogenic organisms in the environmentThe authors thank the relevant fellows of the national tilapia seed farm in Guangdong Province for providing sampling action and the laboratory facilities for virulence test We also thank all the people who have dedicated their time to these experiments
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