Authors: Xue Yan Aurélie BudinVerneuil Nicolas Verneuil Michael S Gilmore Sébastien Artigaud Yanick Auffray Vianney Pichereau
Publish Date: 2014/09/23
Volume: 70, Issue: 2, Pages: 156-168
Abstract
Enterococcus faecalis is a Grampositive commensal bacterium inhabiting the gastrointestinal tracts of human and other mammals but is also increasingly recognized as an opportunistic human pathogen Oxidative stress is one of the major challenges encountered by enterococci both in their natural environment and during infection In this paper we evaluated the transcriptomic response of E faecalis to oxidative stress and showed that transcript abundance was reduced for 93 genes and increased for 39 genes during growth in medium containing 175 mM H2O2 The presence of hydrogen peroxide affected several metabolic pathways including a large decrease in ethanolamine utilization and methylglyoxal metabolism and an increase in transcript abundance for several transport systems In particular four operons encoding iron transporters appeared highly induced By contrast in our experimental conditions the expression of most of the genes known to be involved in the enterococcal response to oxidative stress did not appear significantly alteredXue Yan was the recipient of a doctoral fellowship from INRA MICA Department and the Conseil Régional de BasseNormandie France Microarray design development and production was supported by NIH Grant AI083214 “Harvardwide program on antibiotic resistance” The authors deeply thank Annick Blandin and MarieJeanne Pigny for expert technical assistance and Christelle Thibault from IGBMC Strasbourg France for DNA chip hybridization
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