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Title of Journal: Curr Microbiol

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Abbravation: Current Microbiology

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Springer-Verlag

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DOI

10.1007/bf01752359

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1432-0991

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SUMO Mediating Fusion Expression of Antimicrobial

Authors: Jian Feng Li Jie Zhang Zhen Zhang Chun Tao Kang Shuang Quan Zhang
Publish Date: 2010/07/17
Volume: 62, Issue: 1, Pages: 296-300
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Abstract

Antibacterial peptide CM4 ABPCM4 is a small cationic peptide with broadspectrum activities against bacteria fungi and tumor cells which may possibly be used as an antimicrobial agent To improve the expression level of CM4 in Escherichia coli two tandem repeats of CM4 genes were cloned into the vector pSUMO to construct an expression vector pSUMO–2CM4 The fusion protein SUMO–2CM4 purified by Ni2+chelating chromatography was cleaved by hydroxylamine hydrochloride to release recombinant CM4 After the cleaved sample was reapplied to a NiIDA column finally about 48 mg recombinant CM4 was obtained from 1 L bacterial culture with no less than 96 purity which was the highest yield of CM4 reported so farThis work was supported by the Grants of Nanjing Normal University and Jiangsu Province Graduate Innovation Project No CX07S020z administered by Prof Zhang This work was financially supported by National Nature Science Foundation of China No 30270193 and Natural Science Foundation of Jiangsu Province China No BK2006221

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