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Title of Journal: Curr Microbiol

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Abbravation: Current Microbiology

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Springer US

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DOI

10.1016/0038-1098(72)90500-5

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1432-0991

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Multiplex RTPCR and Indirect Immunofluorescence A

Authors: Manel Ben M’hadheb Myriam Harrabi Amira Souii Nadia JradBattikh Jawhar Gharbi
Publish Date: 2014/11/01
Volume: 70, Issue: 3, Pages: 324-329
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Abstract

Influenza viruses are negative stranded segmented RNA viruses belonging to Orthomyxoviridae family They are classified into three types A B and C Type A influenza viruses are classified into subtypes according to the antigenic characters of the surface glycoproteins hemagglutinin H and neuraminidase N The aim of the present study is to develop a fast and reliable multiplex RTPCR technique for detecting simultaneously the subtypes A/H1N1 and A/H3N2 of influenza virus Our study included 398 patients mean age 3033 ± 1992 years with flu or flulike syndromes consulting physicians affiliated with collaborating teams A multiplex RTPCR detecting A/H1N1 and A/H3N2 influenza viruses and an examination by indirect immunofluorescence IFI were performed In the optimized conditions we diagnosed by IFI a viral infection in 90 patients 226  85 cases of influenza type A four cases of influenza type B and only one case of coinfection with types A and B An evaluation of the technique was performed on 19 clinical specimens positive in IFI and we detected eight cases of A/H3N2 five cases of A/H1N1 one case of influenza virus type A which is not an H1N1 nor H3N2 and five negative cases Multiplex RTPCR is a sensitive technique allowing an effective and fast diagnosis of respiratory infections caused by influenza viruses in which the optimization often collides with problems of sensibility

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