Authors: Behtash Ghazi Nezami Sina Rahimpour Taha Gholipour Seyedmehdi Payabvash Reza Rahimian Seyed Mohammad Tavangar Seyed Hassan EmamiRazavi Ahmad Reza Dehpour
Publish Date: 2008/09/03
Volume: 41, Issue: 1, Pages: 93-99
Abstract
Sprague–Dawley rats were divided into five groups with ten animals in each In animals undergoing torsion/detorsion right testes were rotated 720º for 1 h A baseline group was for basal normal values The shamoperated group served as a control group The TD group underwent torsion/detorsion surgery alone the cyclosporineA group TDCsA received intravenous cyclosporine injection 5 mg/kg at the time of detorsion and the FK506 group TDFK received intravenous FK506 35 mg/kg at the time of detorsion For measurement of lipid peroxidation and antioxidant enzyme activities the right testes of five animals in each group were excised after 4h reperfusion Germ cell apoptosis indices were determined 24 h following detorsion in the right testes of the remaining five animals in each groupMalondialdehyde MDA levels in the TD group were significantly higher compared to control and baseline groups Moreover testicular MDA values in TDCsA and TDFK groups were significantly lower than in TD There were also significant decreases in catalase and superxide dismutase activities in the TD group compared to control and baseline groups These values in TDCsA and TDFK groups were significantly higher than in TD The mean germ cell apoptosis scores were significantly higher in TD animals compared to control and baseline groups however CsA and FK506 treatment significantly reduced the apoptosis compared with the TD groupWe have shown that administration of immunophilin ligands in testicular torsion decreases ischemia/reperfusion I/R cellular damage The results of biochemical studies suggest that reduction of oxidative stress along with attenuated neutrophil accumulation by immunophilin ligands may have a major role in their cytoprotective effects
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