Authors: YungShun Juan Anita Mannikarottu Shu Mien Chuang Suning Li Alpha D Lin Lan ChangChou Catherine Schuler Robert E Leggett Robert M Levin
Publish Date: 2009/09/17
Volume: 42, Issue: 3, Pages: 637-645
Abstract
Obstructive bladder dysfunction is directly related to ischemia/reperfusion injury characterized by damage to nerves synapses and smooth muscle cells within the bladder wall Antrodia Camphorata AC has significant antioxidant antiinflammatory and cellcycle inhibition properties The specific aim of this study was to evaluate whether orally administered AC can protect rabbit bladders from the progressive dysfunctions induced by bilateral ischemia/reperfusion I/RTwentyfour male NZW rabbits were separated into 4 groups of 6 animals each Rabbits in groups 1 and 2 were fed Antrodia Camphorata AC suspensions those in groups 3 and 4 received vehicle Each rabbit in groups 2 and 4 were subjected to in vivo bilateral ischemia for 2 h and then allowed to recover for 1 week The rabbits in groups 1 and 3 received sham operation and served as control groups Cystometry contractile responses to field stimulation carbachol ATP and KCl were determined Biochemical and immunohistochemical studies were also performedI/R resulted in decreased compliance decreased contractile responses decreased nerve density and increased apoptosis AC pretreatment of rabbits subjected to I/R significantly protected the bladder from all contractile biochemical and structural dysfunctions resulting in significantly improved bladderThis material is based upon work supported in part by a generous grant from the Wellshine Biotech Company Taipei Taiwan in part by the Office of Research and Development Medical Research Service Department of Veteran’s Affairs and in part by NIH grant RO1DK 067114
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