Authors: Cynthia Jongen Kora de Bruin Freek Beekman Jan Booij
Publish Date: 2008/04/19
Volume: 35, Issue: 9, Pages: 1692-
Abstract
The dopamine D2 receptor D2R is important in the mediation of addiction 123Iiodobenzamide IBZM a SPECT ligand for the D2R has been used for in vivo studies of D2R availability in humans monkeys and rats Although mouse models are important in the study of addiction 123IIBZM has not been used in mice SPECT studies This study evaluates the use of 123IIBZM for measuring D2R availability in micePharmacokinetics of 123IIBZM in mice were studied with pinhole SPECT imaging after intravenous iv injection of 123IIBZM 20 40 and 70 MBq In addition the ability to measure the release of endogenous dopamine after amphetamine administration with 123IIBZM SPECT was investigated Thirdly iv administration the standard route of administration and intraperitoneal ip administration of 123IIBZM were comparedSpecific binding of 123IIBZM within the mouse striatum could be clearly visualized with SPECT Peak specific striatal binding ratios were reached around 90 min postinjection After amphetamine administration the specific binding ratios of 123IIBZM decreased significantly −272 n = 6 p = 0046 Intravenous administration of 123IIBZM led to significantly higher specific binding than ip administration of the same dose However we found that iv administration of a dose of 70 MBq 123IIBZM might result in acute ethanol intoxication because ethanol is used as a preparative aid for the routine production of 123IIBZM
Keywords: