Authors: Sofie Bæk Christlieb Casper Nørgaard Strandholdt Birgitte Brinkmann Olsen Karen Juul Mylam Thomas Stauffer Larsen Anne Lerberg Nielsen Max Rohde Oke Gerke Karen Ege Olsen Michael Boe Møller Bjarne Winther Kristensen Niels Abildgaard Abass Alavi Poul Flemming HøilundCarlsen
Publish Date: 2016/04/22
Volume: 43, Issue: 10, Pages: 1824-1836
Abstract
The purpose of this study was to determine the ability of dual timepoint DTP PET/CT with 18FFDG to discriminate between malignant and benign lymphadenopathies The relationship between DTP FDG uptake and glucose metabolism/hypoxia markers in lymphadenopathies was also assessedPatients with suspected lymphoma or recently diagnosed treatmentnaive lymphoma were prospectively enrolled for DTP FDG PET/CT scans 60 min and 180 min after FDG administration FDGavid nodal lesions were segmented to yield volume and standardized uptake values SUV including SUVmax SUVmean cSUVmean with partial volume correction total lesion glycolysis TLG and cTLG with partial volume correction Expression of glucose transporter1 GLUT1 hexokinaseII HKII glucose6phosphatase G6Pase and hypoxiainducible factor1alpha HIF1alpha were assessed with immunohistochemistry and enzyme activity was determined for HK and G6PaseFDG uptake was assessed in 203 lesions 146 malignant and 57 benign Besides volume there were significant increases over time for all parameters with generally higher levels in the malignant lesions The retention index RI was not able to discriminate between malignant and benign lesions Volume SUVmax TLG and cTLG for both scans were able to discriminate between the two groups statistically but without complete separation Glucose metabolism/hypoxia markers were assessed in 15 lesions TLG and cTLG were correlated with GLUT1 expression on the 60min scan RImax and RImean and SUVmax SUVmean and cSUVmean on the 60min scan were significantly correlated with HKII expressionWe would like to thank the Departments of Nuclear Medicine Haematology Ear Nose and Throat and Pathology of Odense University Hospital for making this study possible Special thanks are due to Marianne Knudsen and Henrik Petersen for making the recruiting of patients possible and helping with all the logistic challenges to Christian Godballe and the secretaries of the Department of Ear Nose and Throat for helping with the recruitment of patients to Ole Nielsen and Lisbet Mortensen of the Department of Pathology for immunohistochemistry staining of the samples and to the physicists of the Department of Nuclear Medicine for helping with technical difficulties Last but not least we thank the Danish Council for Independent Research for funding this study grant no 409200378BAll procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the principles of the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards
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