Authors: Jonathan M DuBois Olivier G Rousset Jared Rowley Manuel PorrasBetancourt Andrew J Reader Aurelie Labbe Gassan Massarweh JeanPaul Soucy Pedro RosaNeto Eliane Kobayashi
Publish Date: 2015/08/21
Volume: 43, Issue: 1, Pages: 152-162
Abstract
Metabotropic glutamate receptor type 5 mGluR5 is a G proteincoupled receptor that has been implicated in several psychiatric and neurological diseases The radiopharmaceutical 11CABP688 allows for in vivo quantification of mGluR5 availability using positron emission tomography PET In this study we aimed to detail the regional distribution of 11CABP688 binding potential BPND and the existence of age/sex effects in healthy individualsThirtyone healthy individuals aged 20 to 77 years men n = 18 453 ± 182 years females n = 13 415 ± 196 years underwent imaging with 11CABP688 using the highresolution research tomograph HRRT We developed an advanced partial volume correction PVC method using surfacebased analysis in order to accurately estimate the regional variation of radioactivity BPND was calculated using the simplified reference tissue model with the cerebellum as the reference region Surfacebased and volumebased analyses were performed for 39 cortical and subcortical regions of interest per hemisphereWe found the highest 11CABP688 BPND in the lateral prefrontal and anterior cingulate cortices The lowest 11CABP688 BPND was observed in the pre and postcentral gyri as well as the occipital lobes and the thalami No sex effect was observed Associations between age and 11CABP688 BPND without PVC were observed in the right amygdala and left putamen but were not significant after multiple comparisons correctionThe study was funded by the Savoy Foundation for Epilepsy wwwsavoyfoundationca pilot project grant to EK and PRN and PhD studentship to JMD and partially by the American Epilepsy Society wwwaesnetorg Early Career Physician Scientist Award to EK Canadian Institutes of Health Research CIHR wwwcihrirscgcca MOP115131 to PRN and MOP93614 to EK and the Fonds de la recherche en santé du Québec wwwfrqsgouvqcca PRN research fellowAll procedures performed in studies involving human participants were in accordance with the ethical standards of the Montreal Neurological Institute Research Ethics Board and the institutional review board of McGill University and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards This article does not contain any studies with animals performed by any of the authorsSimplified representation of volumetosurface sampling method a Left hemisphere shown in the coronal plane of a single subject’s T1 MRI b Enlarged view of the anterior portion of the temporal lobe A white line denotes the white matter surface and a blue line denotes the pial surface An RBVPVC BPND image in yellowred color scale is coregistered and superimposed over the T1 MRI The black dotted line represents corresponding vertices with the midpoint indicated by the black circular point In this example BPND values are sampled from the midpoint to the surface mesh The vertex corresponding to the midpoint is denoted by the green circle on the magnified surface mesh c The smaller white box denotes the region of the anterior temporal lobe that the magnified surface mesh corresponds to RBVPVC BPND values for all vertices are shown on the lateral left hemisphere pial surface of the same subject d GIF 464 kb
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