Authors: Nicolas Aide Mélanie Briand Pierre Bohn Soizic Dutoit Charline Lasnon Jacques Chasle Jean Rouvet Romain Modzelewski Antony Vela Edwiges Deslandes Pierre Vera Laurent Poulain Franck Carreiras
Publish Date: 2010/09/30
Volume: 38, Issue: 2, Pages: 323-333
Abstract
Human embryonal carcinoma xenografts six/rat were untreated controls or treated to form mature teratomas with lowdose cisplatin and alltrans retinoic acid ATRA over a period of 8 weeks In another group necrosis was induced in xenografts with highdose cisplatin plus etoposide two cycles18FFluorodeoxyglucose 18FFDG small animal positron emission tomography SA PET imaging was performed in three rats one control and two treated for 4 and 8 weeks with cisplatin+ATRA Imaging of αvβ3 expression was performed in six rats bearing mature teratomas and two rats with necrotic lesions on a microSPECT/CT device after injection of the tracer 99mTcHYNICRGD 6hydrazinonicotinic acid conjugated to cycloArgGlyAspDPheLys Correlative immunohistochemistry studies of human and mouse αvβ3 expression were performedCisplatin+ATRA induced differentiation of the xenografts After 8 weeks some glandular structures and mesenchymal cells were visible in contrast control tumours showed undifferentiated tissues SA PET imaging showed that mature teratoma had very low avidity for 18FFDG mean standardised uptake value SUVmean = 048 ± 005 compared to untreated embryonal carcinoma SUVmean = 092 ± 013 p = 0005 αvβ3 imaging accurately distinguished mature teratoma tumour to muscle ratio = 429 ± 157 from necrosis tumour to muscle ratio = 13 ± 026 p = 00002 Immunohistochemistry studies showed that αvβ3 integrin expression was strong in the glandular structures of mature teratoma lesions and negative in host stromaThis work was supported by a grant from the French Ligue contre le cancer comités du Calvados et de l’Orne The Inveon small animal PET scanner was purchased with grants from the Association pour le recherche sur la cancer ARC the Région Basse Normandie and funds from the François Baclesse Comprehensive cancer centre The αvβ3 microSPECT/CT studies were supported by a grant from GEFLUC Rouen
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