Authors: Hedi Ben Mansour Kamel Ghedira Daniel Barillier Leila Chekir Ghedira Ridha Mosrati
Publish Date: 2011/05/10
Volume: 18, Issue: 9, Pages: 1527-1535
Abstract
Acid orange 52 AO52 extensively used in textile industries was decolorized by Pseudomonas putida mt2 AO52 azoreduction products such as NN′dimethylpphenylenediamine DMPD and 4aminobenzenesulfonic acid 4ABS were identified in the static degradation mixture These amines were identified only in media of static incubation which is consistent with their biotransformation under shaken incubation aerobic conditionsDegradation of DMPD by entire cells led to the formation of catechol These results show that P putida was able to decolorize AO52 and metabolize its derivative amines In addition the ability of tested compounds was evaluated in vitro to reduce human plasma butyrylcholinesterase BuChE activityAzoreduction products seem to be responsible for BuChE inhibition activity observed in static biodegradation extract However toxicity of AO52 completely disappears after shaken incubation with P putida suggesting that bacterium has a catabolism which enables it to completely degrade AO52 and especially to detoxify the dye mixtureThe authors gratefully acknowledge the financial support of the “Agence Universitaire de la Francophonie AUF” Paris France The authors also thank Benoit Basset and Nathalie Duparc Université de Caen BasseNormandie France for their assistance on HPLC analysis
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